The Use of Genus-Specific Amplicon Pyrosequencing ...

2015-3-16 · DNA was extracted from pure cultures using the E.Z.N.A. Plant Kit (Omega Bio-tek, Doraville, USA) according to the manufacturer''s recommendations. Control reaction Each of the genomic DNA from cultures was diluted 10, 100 and 1000 times and amplified separately using a SYBR green real-time PCR assay with the Phytophthora -specific primers ...

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Company Information

2014-6-17 · All tissue of each individual (i.e. the whole plant including the stem bark and xylem, shoots, leaves and roots) was separately sampled, immediately frozen in liquid nitrogen, and stored at −80 °C until use. Total RNA of each sample was extracted using an E.Z.N.A. Plant RNA Kit (OMEGA Bio-Tek, USA) according to the manufacturer''s protocol.

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Frontiers | Genome Synteny Has Been Conserved …

Genomic DNA was extracted from immature leaf tissue using the E-Z 96 Plant DNA kit (Omega Bio-Tek, Norcross, GA, USA) with Proteinase K was added to the initial buffer, and RNase treatment delayed until lysate was removed from the cellular debris. An …

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Arbuscular Mycorrhizal Fungus and Exogenous Potassium ...

2021-9-8 · Salt stress is one of the major abiotic stress, impedes plant photosynthetic processes, changes root architecture to impact leaf water status, and reduces potassium uptake and K+/Na+ ratio. Arbuscular mycorrhizal (AM) fungus and extra potassium promote plants tolerance of salt stress, respectively. However, little is known about the combined influence of AM fungus and extra potassium …

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Transcriptome comparison reveals the patterns ...

2014-6-17 · Plant material and RNA extraction. The wild B. nivea var. tenacissima (QYZM) grown in the experimental field of the Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China, was used for cutting propagation. Four 60-day-old cuttage seedlings of QYZM were used for RNA sequencing as four replicates. All tissue of each individual (i.e. the whole plant including …

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Tandem fluorescent protein timers ...

2012-6-24 · Yeast strains were grown in SC-glucose medium at 30 °C and immobilized in glass bottom dishes (P35G-1.5-14-C, MatTek) or 8-well chambers (Lab-Tek 155409, Nunc) for 30 min before imaging.

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2013-7-25 · Nakayama Stone Crusher Machine,Nakayama Mobile Stone Crusher,Price of … a 50 tonnes per hour production capacity.With an abundance of … Chart; Stone Crusher. concrete plants for sale in texas in United States – Gold Ore …

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Specific distribution of gibberellins ...

1997-1-1 · Plant, Physiol., Wll151., pp.J 51-59d (1997)J Plant Physiol. Wll151. pp. 51-59 (1997) A. N. KARA, A. A. KOTOV, and N. G. BUKHOV IntroductionIntroduction Ind higherd plants,d matured leavesd actd asd ad metabolicd source In higher plants, mature leaves act as a metabolic source supportingd thed developmentd ofd sinkd organd byd ...

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Pyrethric acid of natural pyrethrin insecticide: complete ...

2019-3-28 · Introduction. The pyrethrum plant (Tanacetum cinerariifolium, family Asteraceae) and related Tanacetum species synthesize a class of compounds called pyrethrins that serve as efficient insecticides, particularly against flying insects (Casida, 1973).Pyrethrins have been used by people for pest control since ancient times because they are harmless to people and most vertebrates (Casida, 1973 ...

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Company Information

Nikon X-Tek Systems Ltd. Tring Business Centre, Icknield Way, Tring, Herts. HP23 4JX. UK Tel: +44-1442-828-700 Main businesses: Development and manufacturing of X-ray CT, and manufacturing of …

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2017-8-10 · The Arabidopsis LAZY1 Family Plays a Key Role in Gravity Signaling within Statocytes and in Branch Angle Control of Roots and Shoots. Plant & Animal Science. 2017801. The Plant Cell. Masatoshi Taniguchi, Masahiko Furutani, Takeshi Nishimura, Moritaka Nakamura, Toyohito Fushita, Kohta Iijima, Kenichiro Baba, Hirokazu Tanaka, Masatsugu ...

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BdWRKY38 is required for the incompatible ...

2020-9-5 · The B. distachyon accessions Bd21, Bd3-1 and Tek-3 were originally obtained from the National Plant Germplasm System of the USDA-ARS. Brachipodium distachyon seeds of wild-type and transgenic plants were germinated on a moist filter paper and 1/2 MS medium containing 1% (w/v) agar and hygromycin B (25 μg ml −1 ), respectively.

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Secretion dynamics of soyasaponins ...

2020-9-6 · 1 INTRODUCTION. The term "rhizosphere" was coined by L. Hiltner in 1904 and refers to "the zone of soil surrounding the root which is affected by it" (Hartmann, Rothballer, & Schmid, 2008, Hiltner, 1904).Plant roots function as an anchor that supports the plant body and absorb nutrients and water; they also secrete a variety of plant-derived metabolites into the rhizosphere, which ...

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BdWRKY38 is required for the ...

BdWRKY38 is required for the incompatible interaction of Brachypodium distachyon with the necrotrophic fungus Rhizoctonia solani Yusuke Kouzai 1,2, Minami Shimizu, Komaki Inoue 1, Yukiko Uehara-Yamaguchi, Kotaro Takahagi1,3, Risa Nakayama, Takakazu Matsuura4, Izumi C. Mori4, Takashi Hirayama4, Sobhy S. H. Abdelsalam5, Yoshiteru Noutoshi5,* and Keiichi Mochida1,2,3,4,6,*

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Efficacy of Traps, Lures, and Repellents ...

2012-2-1 · The black twig borer, Xylosandrus compactus (Eichhoff) (Coleoptera: Curculionidae: Scolytinae), originally is from Asia and is one of the most serious pests in coffee plantations as well as a number of endemic forest trees in Hawaii (Hara and Beardsley 1979, Marsden 1979). Xylosandrus compactus attacks >200 plant species including agricultural crops such as cacao (Theobroma cacao …

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Characterization of Cadmium Accumulation in Willow as a ...

2010-4-8 · The stems were cut from the plants and embedded in an OCT compound (Sakura Tissue-Tek, Tokyo, Japan) and frozen in liquid nitrogen. Transverse sections of the stems were cut to a thickness of 50 μm using a stainless steel blade (35A, Feather, Osaka, Japan) attached to a cryomicrotome (Leica 3050S, Wetzlar, Germany) at −20°C.

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のにするのみを | …

2020-9-8 · のにするのみを. -のにつ-. ()センターバイオチームの、チームリーダーと( …

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nakayama crushers | Mining & Quarry Plant

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Extraction of Natural Rubber from ...

The authors have developed and optimized a method for extracting rubber from dry plant tissue using polar solvents (water and acetone), with the final extraction with a non‑polar solvent (hexane ...

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Arbuscular mycorrhizal fungi alter carbohydrate ...

2020-3-1 · Recently, mycorrhizal plants have been considered to be potential candidates for phytoremediation (Lenoir et al., 2016; Ma et al., 2019). The main benefit of AM fungi to plants is improved absorption of nutrients such as phosphorus and nitrogen; in return, host plants provide organic carbon to AM fungi (Zhang et al., 2016).

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